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Trpzyme® 重组胰蛋白酶消化液 10X 不含酚红，不含动物源成分

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Catalog Number：S340KJ
Size：500 mL

Product Description

本品采用微生物发酵表达而获得的高纯度、高比活重组胰蛋白酶作为主要原料，通过无菌过滤配制而成的细胞消化液，产品中不使用任何含动物源的原料，产品受外源病毒污染的风险极低，具有极高的生物安全性，可完全代替猪源或牛源胰蛋白酶（Trypsin）在生物工程各个领域中的应用，特别是重组蛋白、疫苗制品生产过程中的哺乳动物细胞的消化，同时无需使用胰蛋白酶抑制剂来中和细胞消化后残留在培养基里的微量酶活。

其主要用途有：

1. 用于疫苗生产过程中宿主细胞的消化；

2. 干细胞、原代细胞传代过程中的消化；

3. 重组蛋白的生产过程中贴壁CHO细胞的消化。

与传统胰酶相比具有如下优势：

1. 可应用于含血清和无血清培养基，适用于多种细胞；

2. 稳定，可以在冷藏环境下（2~ 8 ℃）长期使用保存；

3. 温和，对细胞损伤最小；

4. 细胞传代后，无需额外添加胰蛋白酶抑制剂中和，用培养液稀释后即可；

5. 消化后细胞形态和增值能力恢复良好，可以得到形态均一的单细胞悬液。

注意：该产品为10X浓度溶液，使用前请使用不含钙镁离子的DPBS稀释到1X后使用，我们还提供1X的即用型产品，请参见货号S342KJ。

本产品使用注射用水（Water-For-Injection）配置。

本产品关注点

不含（-）

• 酚红

• 动物源成分

本产品供科学研究和生产使用，用于组织和细胞的体外培养。禁止临床使用。

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    • Quality Assurance

    Products from Shanghai BasalMedia Technologies Co.,LTD are all manufactured in cGMP-compliant facilities. Shanghai BasalMedia Technologies Co.,LTD is certified with ISO9001:2015 and ISO13485:2016 quality system standards.

    Manufacturing Assurance

    We have been committed to adopting modern management models to make all production and operation activities, including safety production management, scientific, standardized and legalized. It comprehensively promotes the occupational health and safety management system in a scientific way to create a healthy, safe and friendly working environment for every employee of BasalMedia Technologies. We have passed the ISO45001:2018 Occupational Health and Safety Management System Certification and ISO14001:2015 Environmental Management System Certification.
    Catalog Number Size Price (CNY) Availability
    S340KJ 500 mL 4128.00
    S340JV 100 mL 926.00

    Specifications

    Application Area 细胞培养相关试剂
    Product Category 细胞消化与解离试剂
    Form 液体
    Shelf Life 36个月
    With/Without Additives（+） [+]1mM EDTA
    With/Without Additives（-） [-]酚红
    Endotoxin ≤10 EU/mL
    Osmolality 300～380 mOsmol/kg
    pH Level 7.0～7.4
    Storage Conditions 2~8 ℃
    Classification 无动物源
    Shipping Conditions 蓝冰
    Usage 10X

    Documents

    Manuals
    Animal Origin Free Declaration
    Material Safety Data Sheets

    Certificates of Analysis

    Cross Reference Table

    Quality Assurance

    Products from Shanghai BasalMedia Technologies Co.,LTD are all manufactured in cGMP-compliant facilities. Shanghai BasalMedia Technologies Co.,LTD is certified with ISO9001:2015 and ISO13485:2016 quality system standards.

    Manufacturing Assurance

    We have been committed to adopting modern management models to make all production and operation activities, including safety production management, scientific, standardized and legalized. It comprehensively promotes the occupational health and safety management system in a scientific way to create a healthy, safe and friendly working environment for every employee of BasalMedia Technologies. We have passed the ISO45001:2018 Occupational Health and Safety Management System Certification and ISO14001:2015 Environmental Management System Certification.
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